Role of maspin in cancer
© Berardi et al.; licensee Springer. 2013
Received: 29 November 2012
Accepted: 28 January 2013
Published: 7 March 2013
Maspin (mammary serine protease inhibitor), is a member of the serine protease inhibitor/non-inhibitor superfamily. Its expression is down-regulated in breast, prostate, gastric and melanoma cancers but over-expressed in pancreatic, gallbladder, colorectal, and thyroid cancers suggesting that maspin may play different activities in different cell types. However, maspin expression seems to be correlated with better prognosis in prostate, bladder, lung, gastric, colorectal, head and neck, thyroid and melanoma cancer. In breast and ovarian cancer maspin significance is associated with its subcellular localization: nucleus maspin expression correlates with a good prognosis, whilst in pancreatic cancer it predicts a poor prognosis. Since tumor metastasis requires the detachment and invasion of tumor cells through the basement membrane and stroma, a selectively increased adhesion by the presence of maspin may contribute to the inhibition of tumor metastasis. Furthermore the different position of maspin inside the cell or its epigenetic modifications may explain the different behavior of the expression of maspin between tumors. The expression of maspin might be useful as a prognostic and possibly predictive factor for patients with particular types of cancer and data can guide physicians in selecting therapy. Its expression in circulating tumor cells especially in breast cancer, could be also useful in clinical practice along with other factors, such as age, comorbidities, blood examinations in order to select the best therapy to be carried out. Focusing on the malignancies in which maspin showed a positive prognostic value, therapeutic approaches studied so far aimed to re-activate a dormant tumor suppressor gene by designed transcription factors, to hit the system that inhibits the expression of maspin, to identify natural substances that can determine the activation and the expression of maspin or possible “molecules binds” to introduce maspin in cancer cell and gene therapy capable of up-regulating the maspin in an attempt to reduce primarily the risk of metastasis.
Further studies in these directions are necessary to better define the therapeutic implication of maspin.
KeywordsMaspin Serine protease Prognosis
Maspin (mammary serine protease inhibitor), is a member of the serine protease inhibitor/non-inhibitor superfamily (serpin), like plasminogen activator inhibitors 1 and 2 and α1-antitrypsin. Maspin gene is located on chromosome 18q21.3–q23 and it was identified for the first time in 1994.
Maspin expression is down-regulated in breast, prostate, gastric and melanoma cancers but over-expressed in pancreatic, gallbladder, colorectal, and thyroid cancers suggesting that maspin may play different activity in different cell types. These conflicting observations might be explained by distinct subcellular localization of maspin in cancer cells (cytoplasmic, nuclear or both cytoplasmic-nuclear expression); by interactions with extracellular matrix and its structure and epigenetic modifications [1–4].
A characteristic feature of serpin structure, is a reactive center loop (RCL), a peptide stretch that is located 9–15 residues amino-terminal to the reactive site peptide bond. RCL allows the reactive site to present an optimal configuration for binding, and subsequent inhibiting target protease.
Another interesting aspect of Maspin pertains to the G α-helix (G-helix), an internal salt bridge or the P1 position of the reactive center loop. The Maspin G-helix is capable of an “open and closed” conformational change inducing redistribution of charged residues within the molecule.
An intact G-helix is absolutely required for the effect of maspin on cell migration, and the effect of the maspin protein can be mimicked by a short peptide corresponding to this structural element. Maspin and the G-helix in isolation are reliant on α1 integrins for their effects on cell migration .
In fact several studies showed that Maspin elicits changes in the expression of proteins associated with the actin cytoskeleton that predict a less motile and invasive phenotype and reduced metastatic spread. Moreover, RCL appears to mediate binding to a cell surface receptor that promotes cell adhesion to type I collagen and fibronectin [12, 13].
To confirm this maspin action time-lapse video microscopic studies showed that recombinant maspin dramatically also inhibited the lamellopodia extension and vectorial translation .
During the process of metastasis, there are consistent changes in gene expression. Studies of genes that are reduced or silenced have yielded surprising insights into in vivo mechanisms of regulating tumor metastasis. This review describes a tumor suppressor gene, Maspin, which is often silenced in cancer cells and exhibits suppressing activity against tumor growth and metastasis. Maspin has been shown to be involved in processes that are important to both tumor growth and metastasis such as cell invasion, angiogenesis, and more recently apoptosis. Hence, many efforts have been devoted to deciphering the molecular mechanism of maspin. While some insights have come from the protease inhibitory effect of maspin, more perceptive results on how maspin may function in suppressing tumor metastasis have come from studies of gene manipulation, protein interactions and global protein profiling .
Recent evidence indicates, however, that nuclear localization of maspin in cancer cells is necessary for its tumor suppressor activity and nuclear-localized maspin binds to chromatin are required to effectively prevent cells from metastasizing .
About subcellular distribution, Maspin is predominantly cytoplasmic but it also localizes to other cellular compartments and is secreted. Secreted Maspin could bind to extracellular matrix components. Alternatively, it is possible that maspin exerts its role only in the nucleus at the level of gene or chromatin regulation and thus indirectly affects the cell-matrix interaction or differentiation state and is released only as a consequence of cell damage or necrosis [20–27].
It has recently established that maspin is epigenetically regulated in tissue-specific way. Epigenetic changes of maspin expression involve cytosine methylation, histone, deacetylation, and chromatin accessibility causing loss of gene function [28–30].
Several studies showed that over-expression of maspin in gastric, pancreatic, and ovarian cancers results from promoter CpG demethylation. This clearly indicates that both methylation and demethylation of maspin promoter could regulate maspin gene expression and could guide the interpretation of overexpression/down regulation associated with negative prognosis. The promoter methylation of the maspin gene leads to gene silencing in several tumors such as breast, thyroid, skin, and colon cancer and it has been recognized as one of the most frequent mechanisms causing loss of gene function. It is noteworthy that in somatic tissues, the majority of CpG islands are methylated, and tumor cells have global DNA hypomethylation compared with their normal counterparts. Hypomethylation is involved in the progression from the premalignant to a fully developed malignancy and leads to activation of genes important for cancer development .
Maspin is the only pro-apoptotic serpin implicated in apoptosis regulation in breast cancer. The intracellular maspin can translocate to the mitochondria to induce cytochrome c release and caspase activation or modulate expression of Bcl-2 family members [32–40].
In order to confirm maspin tumor suppressor function, several authors investigated in vitro maspin expression in different tissue, from normal gland to metastatic disease. Maspin expression appears to be reduced in advanced stages of breast cancer. In fact, a significant stepwise decrease in maspin expression (and in vascular endothelial growth factor (VEGF) expression) occurred in the sequence DCIS (Ductal Carcinoma In Situ)- invasive cancer – brain-lymph-node-bone metastasis experiments. Myoepithelium, normal breast and fibrocystic change showed a strong maspin expression [41–43].
Even if Maspin absence emerges as an indicator of tumor progression and metastatic potential, recent studies showed that maspin expression was correlated with an aggressive phenotype in the breast cancer and with poor prognosis. Three hypothesis were thought for the aberrant expression of maspin in breast cancer cells: maspin gene alteration with loss of activity; a high intracellular density of maspin resulting in auto-inhibition of its function; myoepithelial cell differentiation in cancer cells could contribute to more aggressive phenotype. It is also important to investigate the different subcellular maspin expression. In fact nuclear staining was demonstrated to be significantly associated with better a prognosis than cytoplasmic staining [44, 45].
Umekita et al. and Kim et al. examined, 92 and 192 invasive ductal carcinomas respectively. They reported that maspin expression was frequently observed in invasive ductal carcinoma with an aggressive phenotype (i.e. high histological grade), and it was a strong indicator of a poor prognosis [46, 47].
Conversely, in order to define the significance of subcellular maspin location, Mohsin et al. performed a preliminary study assessing the associations of maspin with other established prognostic factors in invasive breast cancer. In a series of 1068 breast cancer maspin nuclear staining was significantly associated with good prognostic factors rather than cytoplasmic staining .
Furthermore the prognostic role of maspin was investigated by Umekita et al. and Lee et al. They examined maspin and p53 expression in 168 and 80 patients with invasive ductal carcinoma, respectively. Large tumor size, high histologic grade, positive p53 status, negative estrogen receptor or progesterone receptor status and poor prognosis were correlated with maspin expression [49–51].
Stark et al. and Maas et al., instead, investigated the potential correlations between maspin expression in primary tumor and in the metastatic sites. They showed that maspin expression is reduced in primary tumors and again decreased in metastasis [52, 53].
Maspin expression in breast cancer
Clinical pathological features/prognosis
Umekita et al.
92 (invasive breast cancer)
18.5% positive maspin
Positive maspin in invasive breast cancer and DCIS = large tumor size, presence of comedo-necrosis and high grade
145 (Ductal Carcinoma In Situ)
27 (atypical hyperplasia)
3.7% positive maspin
94 (usual hyperplasia)
Kim et al.
192 (stage I-II breast cancer)
34.4% positive maspin
Positive maspin in all type of invasive breast cancer = high grade
36.4% positive maspin in invasive ductal carcinoma
7.1% positive maspin in invasive lobular carcinoma)
Mohsin at al.
1068 (breast cancer)
35% positive cytoplasm
Positive cytoplasmic maspin = negative ER and PgR, high Mib, aneuploidy
96% positive nucleus
Positive nuclear maspin = positive ER and PgR
Umekita et al.
168 (breast cancer)
27.4% positive maspin
Positive maspin = 17.4% Large tumor size, 43.4% high grade, 65.2% negative ER and PgR, 43.4% positive p53 shorter PFS and OS
72.6% negative maspin
Negative maspin = 9% large tumor size, 19.6% high grade 35.2% negative ER and PgR 20,5% positive p53, better PFS and OS
Umekita et al.
135 (triple negative breast cancer)
85.9% positive maspin
Positive maspin = 43.1% age ≤ 50 years, 80.2% high grade
14.1% negative maspin
Negative maspin = 5.2% age ≤ 50 years, 42.1% high grade
Lee et al.
80 (breast cancer)
31.3% positive maspin
Positive maspin = 24% large tumor size, 52% high grade, 80% negative PgR, Short PFS and OS
68.7% negative maspin
Negative maspin = 7.2% large tumor size, 21.8% high grade, 41.8% negative PgR, better PFS and OS
Stark et al.
16 (metastatic breast cancer)
30% positive maspin in primary tumor
13% positive maspin in brain metastasis
Maass et al.
45 (breast cancer)
64% positive maspin
17.7% of patients develop metastasis = 75% negative maspin
36% negative maspin
Nevertheless, further investigations are needed to clarify the real mechanism of the aberrant expression of maspin in breast cancer, also in order to use it as a prognostic marker in clinical practice.
In human prostate cancer, maspin expression consistently appears to be down-regulated at the critical transition from non-invasive, low grade to high grade prostate cancer. In particular, the loss of basolateral maspin expression in benign secretory cells, the dramatically up-regulation in High Grade Prostatic Intraepithelial Neoplasia and the progressively decrease in invasive cancer are associated with maspin capability to reduce tumor growth, osteolysis and angiogenesis. Furthermore, there is an evidence that maspin inhibits prostate cancer-induced bone matrix remodeling and induces prostate cancer glandular redifferentiation [54–61].
Lovric et al. examined 34 biopsies of prostatic carcinoma. Maspin resulted expressed in the cytoplasm of basal cells of normal prostatic glands, whilst normal luminal cells were inconsistently weakly positive and it was aberrant over-expressed in prostate cancer with a predominance of nuclear presence .
On the other hand, Riddick et al. and Machtens et al. analyzed gene expression and clinico-pathologic features in 44 and 84 patients with prostate cancer, respectively. Maspin expression was inversely correlated with the Gleason score and positively correlated with lower tumor stage, more differentiated grade and a lower p53 protein mutation [63, 64].
Maspin expression in prostate cancer
Lovric et al.
34 prostate cancer
79% positive maspin with nuclear expression
Riddick et al.
44 prostate cancer
Positive maspin = lower Gleason score
Machtens et al.
84 prostate cancer
58% positive maspin
Positive maspin = OS: 78 months, PFS: 41 months, GIII-IV: 23%, N1/N2: 9% OS = 62 months, PFS = 26 months, GIII-IV = 48%, N1/N2 = 18%
18% negative maspin
Zou et al.
97 prostate cancer
40.2% positive maspin
Positive maspin = 91.7% partial response with neoadjuvant therapy
49.8% negative maspin
In conclusion, Maspin expression correlates with a better prognosis and may serve as a biomarker for prostate cancer cells responding to the androgen ablation therapy.
Recent evidences seem to correlate a low maspin expression in bladder tumors to an increased tumor cell growth both in vivo and in vitro. In particular, maspin expression was preserved in superficial bladder cancers but significantly decreased in invasive carcinomas. Within the group of invasive cancers, the authors found that maspin expression was associated with good prognosis.
Recently, Lockett et al. showed the essential role of maspin in epithelial homeostasis and that a sub-cellular location of maspin seems to reflect a distinct tumor progression pathway. In particular, the nuclear location of maspin seems to be associated with lower histological grade and longer recurrence free disease [65–79].
Acikalin et al. evaluated the clinical significance of maspin in patients with T1 bladder cancer. They showed that maspin expression was associated with a longer time to recurrence and progression-free survival (PFS) than maspin-negative group .
Furthermore Kramer et al. investigated the role of maspin in transitional cell carcinoma of the bladder as well as its prognostic impact. Specimens from 162 non-muscle invasive bladder cancer patients treated by transurethral resection were examined. They showed that a low maspin protein expression was correlated with a higher incidence of tumor progression and emphasized a possible clinical role of this novel tumor suppressor gene in transitional cell carcinoma of the bladder .
In another study, Friedrich et al. analyzed the expression patterns of maspin in 110 pTa/pT1 urothelial carcinoma of the bladder and compared them with microvessel density (MVD) evaluated by CD105 and CD34. They found a decreased Maspin expression in a large portion of pTa/pT1 bladder tumors and shorter PFS with an increase of MVD in maspin negative cancers .
Again, Sugimoto et al. evaluated maspin expression in 65 series of bladder cancer. Maspin expression was significantly correlated with the development of muscle invasive bladder cancer. Their contradictory results were explained by the fact that maspin could contribute to bladder cancer development through DNA methylation and histone deacetylation .
Another recent study, focused on the role of Maspin in squamous cell carcinoma (SCC) and transitional cell carcinoma (TCC) of urinary bladder.
In 134 bladder cancer patients, the relationship between clinico-pathological features and Maspin was examined and a high Maspin expression was found in low grade and advanced stage. These results indicated that Maspin expression might predict a better prognosis for bladder carcinoma and it could play a role in tumor progression .
Maspin expression in bladder cancer
Acikalin et al.
68 (T1 bladder cancer)
Positive maspin = longer PFS and less recurrence
Negative maspin = shorter PFS and more recurrence
Kramer at al.
162 (pTa-pT1 bladder cancer)
75.9% positive maspin
Positive maspin = PFS: 46 months
24.1% negative maspin
Negative maspin = PFS: 18 months
Friedrich et al.
110 (pTa-pT1 bladder cancer)
33.6% positive maspin
Positive maspin = PFS: 29 months, MVD: 17.7(CD34) and 6.0 (CD105)per field
Negative maspin = PFS: 23 months, MVD = 21.7 (CD34) and 4.2 (CD105)per field
Sugimoto et al.
65 (22 transurethral resection and 43 radical resection specimens)
18.2%positive maspin in transurethral resection specimens
Positive maspin = progression from invasive bladder cancer
51.2% positive maspin in radical resection specimens
Nehad et al.
134 (56 squamous cell carcinoma (scc)and 78 transitional urinary bladder(tcc))
53.7% positive maspin (42.8% scc and 61.5% tcc)
Positive maspin = 91.7% low grade
46.3% negative maspin
Negative maspin = 54.8% high grade
Blandamura et al.
66 (48 pTa e 18 pT1)
38% positive maspin
60.5% positive maspin have high grade
62% negative maspin
22.44% positive maspin have low grade
In conclusion, further studies are needed to define the role of maspin in clinical practice of bladder tumors.
Several studies demonstrated that maspin inhibits the survival pathway by influencing the response to cell death in lung cancer cells .
Bircan et al. investigated maspin in 63 patients with different histological lung carcinoma. The mean percentage of maspin expression was significantly higher in squamous cell carcinoma and adenocarcinoma, than in small cell lung cancer (SCLC) .
Recently, in lung cancer, maspin biological functions have been linked to its subcellular localization. Specifically, a nuclear, opposed to a combined nuclear and cytoplasmic localization has been associated with increased survival in non-small cell lung cancer (NSCLC). Lonardo et al., Frey et al., Woenckhaus et al. and Hirai et al., examined 123 NSCLC, 80 adenocarcinoma, 487 tissue microarrays and 112 specimens, respectively. They found that squamous cell carcinoma showed the highest expression and almost exclusively a combined nuclear-cytosolic stain in early stage. In contrast, nuclear maspin alone, correlated with favorable clinico-pathologic features and improved prognosis especially in adenocarcinoma [88–90].
Recently our group showed that maspin expression, with nuclear or cytoplasmic localization, together with smoking history, represented prognostic factors in NSCLC. In particular a significant longer overall survival (OS) was seen in patients with higher compared with lower expression of nuclear maspin, and poorer OS was present in patients with a higher intensity of cytoplasmic staining .
In another recent study, Wu et al. analysed the expression of maspin in NSCLC and its relationship to vasculogenic mimicry (VM). A total of 160 specimens of NSCLC were considered in this study. The loss of maspin expression may contribute to the invasion and metastasis of NSCLC and it has a positive relationship to VM in NSCLC .
Maspin expression in lung cancer
Clinical features/prognosis/predictive factors
Bircan et al.
28 (squamous cell lung cancer)
89.3% positive maspin
18 (lung adenocarcinoma)
77.8% positive maspin
17 (small cell lung cancer)
52.9% positive maspin
Lonardo et al.
46 (squamous cell lung cancer)
100% positive maspin
Positive maspin = almost exclusively nuclear position
77 (lung adenocarcinoma)
93,5% positive maspin
Positive nuclear maspin in squamous cell lung cancer = low grade, low proliferative rate, absence of invasion, negative p53 vs nuclear-cytoplasmic position.
Frey et al. 2009
80 (lung adenocarcinoma)
93.7% positive maspin
62.6% positive nucleus
Positive nuclear maspin = 36.6% proliferative rate, Stage I OS: 87.7 ± 6.9 months, 42.5% moderate and poor differantation, 25,5% p53+, 4.2% high VEGF
37.3% positive cytoplasm - nucleus
Positive cytoplasmic maspin = 56.9%proliferative rate, Stage I OS: 58.7 ± 6.5 months, 71.4% moderate and poor differantation,53.5% p53+, 39.2% high VEGF
Woenckhaus et al.
487 (tissue microarrays)
72.3% positive maspin
65.3% positive nucleus
Positive nuclear maspin = 63.9% squamous cancer, 16.9%
37.8% positive cytoplasm
Positive cytoplasmic maspin = 78.2% squamous cancer, 6.8% adenocarcinoma adenocarcinoma
Hirai et al.
112 (non-small cell lung cancer)
Positive maspin = 77.8% positive cytoplasm Stage III and 36.2% positive cytoplasm Stage I
44.7% negative maspin
Berardi et al.
439 (non-small cell lung cancer)
85.6% positive maspin
Positive maspin = longer OS
22.8% positive nucleus
Positive nuclear maspin = independent prognostic factor
44% positive cytoplasm
Positive cytoplasmic maspin = especially smokers, lower OS than nuclear position
14.4% negative maspin
Negative maspin = Lower OS
Wu et al.
160 (non-small cell lung cancer)
48.1% positive maspin
Positive maspin = lower vasculogenic mimicry and microvessel density, longer OS, low Stage, low grade, low lymphnode metastasis
51.9% negative maspin
Negative maspin = higher vasculogenic mimicry and microvessel density, lower OS, high Stage, high grade, lymphnode metastasis
20 (normal tissue)
100% positive maspin
Nakagawa et al.
210(non-small cell lung cancer)
73.7% positive maspin in squamous cancer
Positive maspin = 70.1% 5-years OS
26.3%negative maspin in squamous cancer
Negative maspin = 41.5% 5-years OS
Katakura et al.
55 (non-small cell lung cancer)
Positive maspin = 67.7% 5-years OS
Negative maspin = 41.4% 5-years OS
Takanami et al.
181 (non-small cell lung cancer)
65.7% positive maspin in squamous cancer
Positive maspin in squamous cell lung cancer = 52.2% 5-years OS
34.3%negative maspin in squamous cancer
Negative maspin in squamous cell lung cancer = 24% OS 5 years OS
22.8% positive maspin in adenocarcinoma
59.2% negative maspin
An evidence that maspin can inhibit ovarian cancer invasion has been shown in vitro, nevertheless the role of maspin in ovarian cancer remains to be demonstrated. However it seems that maspin expression level is low on normal ovarian surface epithelium, while ovarian cancer cell lines expressed high to low level of maspin expression that is also correlated with shorter survival in patients with epithelial ovarian cancer. Maspin expression was predominantly located in the cytoplasm and occasionally in the nucleus of epithelium and cancer cells [96, 97].
Klasa-Mazurkiewicz at al. examined 168 ovarian tissue and found maspin level significantly higher in patients with borderline tumors and early stages ovarian cancers when compared with healthy tissues those with benign and metastatic tumors. Over-expression of maspin was found to correlate with early stage of disease in non-serous subtypes of ovarian cancer and with a positive response to chemotherapy. A statistically significant longer PFS was observed in women with high as compared with low expression of maspin. Again Moshira et al. examining 68 ovarian specimens: 7 normal tissue, 18 benign, 14 borderline, 46 malignant epithelial ovarian neoplasm, detected the same results about progression disease .
As well as in other tumor types, also in ovarian cancer it is important its localization inside the cell in order to define the role of maspin.
Sopel et al. and Solomon et al. studied 132 and 11 invasive epithelial ovarian carcinomas, respectively. Cytoplasmatic maspin expression was correlated with worse clinico-pathologic features and prognosis rather than rare nuclear maspin expression [99, 100].
Few studies compared maspin with VEGF in ovarian carcinoma. In a recent study, over-expression of maspin, VEGFC, and VEGFD was significantly associated with unfavorable clinico-pathologic features and poor prognosis in 60 ovarian carcinoma tissues. Similar results were obtained by Sood et al. that assessed maspin expression in 104 ovarian tissue specimens .
maspin expression in ovarian cancer
Klasa-Mazurkiewicz et al.
76 (ovarian cancer)
82.9% positive maspin
Positive maspin = OS and PFS longer than maspin negative tumor.
87.2% positive maspin
78.6% positive maspin
53.1% positive maspin
Moshira et al.
46(ovarian cancer) 14(borderline)
65.9% positive maspin = 69% positive cytoplasm, 3.4% positive nucleus and 27.6% positive nucleus and cytoplasm
57.1% positive maspin = 37.5% positive cytoplasm and 62.5% positive nucleus and cytoplasm
100% positive cytoplasm
Sopel et al.
132 (ovarian cancer)
88.6% positive maspin
22% positive nucleus
Positive nuclear maspin = low tumor grade, less distant metastasis, low Figo stage, longer OS
12.9% positive cytoplasm
Positive cytoplasmic maspin = high tumor grade, probably distant metastasis, high Figo stage, shorter OS
Solomon et al.
118 (serous ovarian cancer)
81.4% positive maspin
21.2% positive nucleus
Positive nuclear maspin = lower VEGF and COX-2, OS:1803 days
60.2% positive cytoplasm
Positive cytoplasmic maspin = higher VEGF and COX-2, OS:637 days
18.6% negative maspin
Negative maspin = OS:1146 days
Bolat et al.
60 (ovarian cancer)
88.3% positive cytoplasm
Positive maspin = high VEGF, high grade, high clinical stage, ascite, lymphnode methastasis
11.7% positive nucleus–cytoplasm
Sood et al.
71% positive maspin
Positive maspin = shorter OS, 63.3% high grade and 90% ascite
26.6% positive nucleus
Positive nuclear maspin = longer OS
14 (benign tumor)
Bauerschlag et al.
87 (ovarian cancer)
Positive maspin = OS: 28 months and platinum-therapy resistance
Negative maspin = OS: 57 months
Maspin has been investigated for its hypothetic implication in the cancerogenesis of colorectal cancer, for its probable association with conventional histo-pathological features and for its potential as an independent predictor of survival and response to adjuvant chemotherapy.
Cao et al. investigated the relationship between chronic inflammatory states and neoplasia in 125 specimens included inflammatory bowel disease (IBD) with different grade of dysplasia and also with invasive colorectal cancer. Maspin was found paradoxically over-expressed in both active IBD and colitis-associated dysplasia compared to either inactive IBD or normal colonic mucosa, suggesting a potential role in disease “flare” as well as neoplastic progression .
Focusing on the clinico-pathologic features associated with maspin, Umekita et al. studied expression of maspin in colorectal adenocarcinomas from 104 patients and observed that maspin was significantly correlated with the depth of invasion, higher Dukes’ classification and high-grade tumor budding. These results suggest that the expression of maspin may correlate with the aggressiveness of colorectal adenocarcinomas .
On the other hand, Fung et al. examined 450 resected colorectal cancer finding a stronger expression in right than in left-sided tumors and a stronger expression in high-grade tumors .
Regarding relationship between maspin, other biomarker and a possible target therapy, Gurzu at al. evaluated maspin, p53 and other biomarker expression in 110 cases with colorectal cancer with the aim to correlate maspin with angiogenesis and 5-Fluorouracil (5-FU) therapy. They found a correlation with tumor stage and microsatellite status. Therefore maspin nuclear expression, associated with p53 ones, might be used either to select the high-risk microsatellite stable (MSS) colorectal carcinomas diagnosed in Stage II or those MSI cases which can respond to 5-FU .
In another recent study on 156 colorectal cases, significant correlations between cytoplasmic expression and high tumor grade and between nuclear expression, high tumor budding and worse OS, were shown. These findings suggest a compartment-dependent function of maspin in colorectal cancer, which can be useful in identifying stage II cases with a higher risk for fatal outcome with a possible benefit from adjuvant chemotherapy .
Maspin expression in colorectal cancer
Clinical features/prognosis/predictive factors
Cao et al.
25 (colorectal cancer and IBD)
51 (active chronic IBD)
92% positive maspin
30 (inactive chronic IBD)
43% positive maspin
11% positive maspin
Song et al.
66 (colorectal cancer)
75.5% positive maspin
Positive maspin in colorectal cancer = 44.7% mutant p53 expression, microvessel density = 181.1+/−54.2
24.5% negative maspin
Negative maspin in colorectal cancer = microvessel density = 256.1 +/75.4
91.7% positive maspin
Positive maspin in adenoma = 0% mutant p53 expression
Jiang-tao et al.
50 (colorectal cancer)
62% positive maspin
Positive maspin in colorectal cancer = no association with positive lymphnode, higher Duke’s stage or mutant p53 expression
90% positive maspin
20 (normal mucosa)
Zheng et al.
119 (colorectal cancer)
95% positive maspin
Positive maspin in colorectal cancer = no liver metastasis = 89% positive maspin
118 (normal mucosa)
Umekita et al.
104 (colorectal cancer)
66%positive maspin (15% = T1-T2 and 78.5% = T3-T4)
Positive maspin = 44.2% absent tumor budding, 32.7% Duke’s stage B
Fung et al.
450 (colorectal cancer)
81%positive cytoplasm 80% positive nucleus
Positive maspin = right colon and high-grade tumor
Gurzu et al.
101 (colorecatal cancer)
60% positive maspin
Positive maspin = Stage II-III
Markl et al.
156 (colorectal cancer stage I-II)
48% positive nucleus
Positive nuclear maspin = pT3 OS = 40 months
72% positive cytoplasm
Positive cytoplasmic maspin = pT3 OS = 63 months
Dietmaier et al.
172 (colorectal cancer)
44.4% negative nucleus
Negative nuclear maspin = OS: 79.2%, OS after 5FU: 32.5%
55.6% positive nucleus
Positive nuclear maspin = OS:66.6%, OS after 5FU: 71.7%
24.1% negative cytoplasm
75.9% positive cytoplasm
In human gastric cancer the molecular aspect of carcinogenesis and progression remains elusive. Data are very few but it seems that maspin up-regulation may cause a retarding cell proliferation .
Wang et al. investigated maspin expression in 113 gastric cancer and compared it with clinical parameters, MVD and Caspase-3 expression. Cancer less frequently expressed maspin than normal mucosa and dysplasia. Maspin expression showed a significantly negative correlation with invasive depth, metastasis, Lauren’s and Nakamura’s classification and MVD, but a positive correlation with expression of Caspase-3 in gastric cancer .
maspin expression in gastric, pancreatic and gallbladder cancer
Clinical features/prognosis/predictive factors
Wang et al.
113 (gastric cancer)
50.5% positive maspin
Positive maspin = T4: 35.9%, metastasis: 34.3%, subtype diffuse: 42.1%, undifferentiated: 40%
49.5% negative maspin
Negative maspin = T4: 64.1%, metastasis: 65.7%, subtype diffuse: 57.9%, undifferentiated: 60%
Lee et al.
152 (gastric cancer)
71.7% positive maspin
29.3% negative maspin
Negative maspin = undifferentiated, high stage, metastasis and invasion depth, positive p53
Liu et al.
102 (pancreatic cancer)
98% positive maspin
Maas et al.
24 (pancreatic cancer)
96% positive maspin
100% positive maspin
14% positive maspin
14 (cholelitiasis + intestinal metaplasia)
Kim et al.
101 (gallbladder cancer)
59.4% positive maspin
100% negative maspin
10 (normal gallbladder)
On the basis of all the above mentioned results, Maspin expression could be considered as an effective and objective marker to reveal biological behaviors of gastric cancer and it could become an useful marker in the future.
Unlike other malignant tumors, precancerous pancreatic lesions and pancreatic cancer present up-regulation of maspin gene expression, therefore maspin could be considered a new factor associated with pancreatic cancer. Liu et al. and Maass at al. examined 102 and 29 specimens of pancreatic tissue and demonstrated that more than 90% of cases of ductal adenocarcinoma as well as all high-grade precancerous lesions (PanIN3) were positive for maspin, and normal pancreatic ducts and low-grade precancerous lesions were usually negative for maspin (Table 7) .
These data show that maspin may play an important role in the carcinogenesis, tumor invasion, metastasis, and angiogenesis of pancreatic cancer. Its relationship to carcinoma of the pancreas opens a new angle to the discussion on its function in cancer .
There are few published data focusing on maspin expression in gallbladder cancer, but there is an evidence of maspin implication in cholelithiasis-intestinal metaplasia-dysplasia-carcinoma sequence and in the early step of gallbladder cancerogenesis.
In a recent study on 69 patients with cholelithiasis and 30 patients with gallbladder cancer without cholelithiasis, a positive immunoreactivity for maspin was significantly associated with the presence of intestinal metaplasia in patients with cholelithiasis. These data could support the assumption that intestinal metaplasia of the gallbladder may predispose to gallbladder carcinoma .
Furthermore Kim et al. compared the pattern of maspin expression in 101 early and advanced gallbladder cancers. The positivity of maspin expression was found almost in half of gallbladder cancers, whereas no maspin was expressed in adenomas and normal mucosa of gallbladder (Table 7) .
Head and neck cancer
Maspin seems to be an important prognostic factor also in head and neck cancer. In particular some studies revealed that the subcellular location seems to make the difference. Nuclear expression is a positive prognostic factor rather than cytoplasmic location [119, 120].
Yoshizawa et al. and Iezzi et al. investigated the possible correlation between clinico-pathologic findings and maspin expression in 54 and 89 oral squamous cell carcinoma, respectively. Maspin was associated with a better survival rate, but a negative correlation with type of invasion, T-stage, lymph-node metastasis and differentiation grade was found [121–123].
maspin expression in head and neck cancers
Clinical pathological features/prognosis
Yoshizawa et al.
64.8% positive maspin
Positive maspin = 77.8% OS
35.2% negative maspin
Negative maspin = 29.4% OS
Yoshizawa et al.
64.8% positive maspin
Positive maspin = 100% low grade, 93.3% no lymphnode metastasis, better OS
Iezzi et al.
Positive maspin = low grade, negative lymphnode, high stage(?)
Marioni et al.
58.9% positive maspin
5.3 % positive nucleus
1.8% positive cytoplasm-nucleus
51.8% positive cytoplasm
Positive cytoplasmic maspin = 61% pN0 and 33% pN+
41.1% negative maspin
Marioni et al.
68 (laryngeal cancer)
Positive nucleus = 22.7% lower recurrence and PFS: 44.5 ± 27.5 months
Positive cytoplasm and cytoplasm-nucleus = 44.6% longer recurrence and PFS: 34 ± 27.5 months
In conclusion, maspin may represent an useful marker to identify the potential for progression of head and neck cancer, since lower immunoreactivity is associated with larger tumors and a greater invasive potential. Furthermore in head and neck cancer it is necessary to clarify the mechanisms between maspin expression and progression of tumor in order to apply to clinical applications.
Maspin expression was investigated also in thyroid neoplasms originating in follicular cells. Some studies showed that neither normal follicular cells nor stromal cells expressed maspin. Papillary and follicular carcinomas expressed maspin in significantly higher incidence than those with pure papillary or follicular patterns. These findings indicate that maspin expression is directly associated with prognosis in this setting .
Boltze et al. and Tahany et al. detected maspin in 230 and 63 different histological thyroid tissue, respectively, showing that its expression was typical of papillary thyroid tumor, it was associated with tumor multicentricity, vascular and lymphatic invasion, as well as lymph-node metastasis and it was weakly expressed in follicular cancer and absent in normal tissue and in undifferentiated cancers. Maspin expression is a special feature of papillary thyroid carcinomas; promotor methylation-caused maspin repression plays a major role in gene balance and in the process of tumor determination. Therefore maspin could possibly act as a clinically relevant inhibitor of tumor progression, preventing local invasiveness and further systemic progression of papillary thyroid carcinomas [127, 128].
Thyroid cancer and melanoma
Clinical features/prognosis/predictive factors
Boltze et al.
68 (papillary carcinomas)
70.5% positive maspin
Positive maspin in papillary cancer = 2% p53+, 83% 110 months OS, Recurrence free disease: 60 months
38 (follicular carcinomas)
100% negative maspin
Negative maspin in follicular carcinomas = 80%p53+, 40% 110 months OS, Recurrence free disease :40months
Tahany et al.
Positive maspin = 72% papillary thyroid, 61,1% positive cytoplasm and positive nucleus, 11.1 % positive nucleus and 27.8% positive cytoplasm,
25 papillary carcinoma
71.5% negative maspin
Wada et al.
45 (malignant melanoma)
12.5% positive maspin
87.5% negative maspin
Negative maspin in melanoma = 83% trunk, 89% extremites, 89% acral, 86% lentigo maligna melanoma, 100% nodular melanoma, 75% superficial spreading, 100% thickness 1.0-4.0 and >4 mm, 100% II-III-IV stage
Chua et al.
59.7% positive maspin
Positive maspin = less microvessel density, 78% thin melanoma, 46% thick melanoma
35.1% negative maspin
Negative maspin = high microvessel density, 22% thin melanomas, 54% thick melanoma
In melanoma, Maspin was found to have a tumor-suppressor function. Previous reports showed that early hypermethylation of the Maspin promoter might play a role in silencing Maspin. In particular Maspin promoter activity was significantly increased after the thrombin receptor protease activated receptor-1 (PAR-1) silencing, suggesting that PAR1 negatively regulates Maspin at the transcriptional level Maspin tumor-suppressor gene in the acquisition of the metastatic melanoma phenotype [130–133].
Wada et al. investigated maspin expression in five melanoma cell lines, in a normal human epidermal melanocyte cell line, and in 80 surgically resected tumors. Their results suggest that maspin expression in normal skin melanocytes and melanocytic nevi may be repressed, whereas maspin is aberrantly expressed in a subset of melanoma cells by epigenetic modification. In particular Chua et al. in 77 melanoma samples found maspin presence in the radial growth phase in melanoma, and a lost maspin expression in the transition from the radial growth phase to the vertical growth phase of melanoma (Table 9) [134, 135].
The expression of maspin might be useful as a prognostic and possibly predictive factor for patients with particular types of cancer and data can guide physicians in selecting therapy. Its expression in circulating tumor cells especially in breast cancer, could be also useful in clinical practice along with other factors, such as age, comorbidities, blood examinations in order to select the best therapy to be carried out.
Detecting maspin mRNA amplification by RT-PCR, several authors, as mentioned above, showed maspin in normal breast epithelial cells and in primary and metastatic breast cells, but not in the peripheral blood of healthy donors. Furthermore, they found the presence of circulating maspin-positive cells, potentially neoplastic, in the peripheral blood of patients with breast cancer undergoing conventional-dose chemotherapy. On the basis of these observations, they assessed a possible mobilizing effect of chemotherapy, delivered at standard doses, on mammary cells of potential neoplastic origin. Thus suggesting that the detection of circulating breast cells could have prognostic significance, as it was associated with a higher risk of early relapse or disease progression for patients with limited or stage IV disease, respectively. The results of these studies should be interpreted with caution, and larger studies with longer follow-up are required to definitely establish the clinical usefulness of the test [136–139].
Moreover, maspin expression was found directly correlated with treatment including carboplatin plus vinorelbine combined with radiotherapy in primary head and neck squamous cell carcinoma .
In some cases, the subcellular localization could predict response to chemotherapy. In fact, nuclear maspin expression in patients with stage III colon cancer is associated with response to adjuvant 5-FU-based chemotherapy and could help to select patients whose tumors do not express this molecule that may be candidates for an alternative (non-5-FU-based) adjuvant therapy regimen.
Focusing on the malignancies in which maspin showed a positive prognostic value, therapeutic approaches studied so far aimed to re-activate a dormant tumor suppressor gene by designed transcription factors, to hit the system that inhibits the expression of maspin, to identify natural substances that can determine the activation and the expression of maspin or possible “molecules binds” to introduce maspin in cancer cell and gene therapy capable of up-regulating the maspin in an attempt to reduce primarily the risk of metastasis.
Some authors hypothesized that artificial transcription factors (ATFs), composed of modular zinc finger (ZF) domains and designed to recognize specific sequences in the promoter of a tumor suppressor, would result in a re-expression of the endogenous gene silenced by epigenetic mechanisms in aggressive tumor cells (e.g. in metastatic breast cancer and in non-small cell lung cancer) and also in up-regulation of E-cadherin. Reversal and modification of the tumor suppressor epigenetic state can be achieved by blocking DNA and histone methyltransferases as well as histone deacetylases with different doses of ATFs and chromatin remodeling drugs: the methyltransferase inhibitor 5-aza-2’-deoxycytidine and the histone deacetylase inhibitor suberoylanilide hydroxamic acid. It was found that ATFs act in synergy with inhibitors in reactivating endogenous maspin expression. The strongest synergy was observed with the combination treatment ATF-126 + 5-aza-2’-deoxycytidine + suberoylanilide hydroxamic acid. While drugs can be used to achieve the inhibition of these enzymes in an untargeted manner, the precise mechanisms and the genetic cascades whereby these drugs induce biological changes are currently not completely understood and it seems to depend on cell micro-environment. Furthermore, given that tumor suppressors sustain anti-proliferative and anti-metastatic functions, it is essential to target the native tumor suppressor function in order to develop more effective therapies.
Given the clinical relevance of maspin expression in a variety of epithelial tumors (including breast, prostate, lung and colon cancer), these studies describe a novel approach to target multiple human tumors [141–148].
Recently, PAR-1 has been described to regulate the gap junction protein Connexin 43 and the maspin tumor suppressor gene to promote the metastatic melanoma phenotype. Silencing PAR-1 results in decreased activation of p38 MAPK, allowing for increased binding of c-Jun and Ets-1 transcription factors to the maspin promoter. Increased Maspin expression further inhibits cell invasion, through decreased expression and activity of MMP-2, as well as angiogenesis through decreased VEGF expression .
An important role of IL-6TS in decreasing adhesion and increasing motility and migration in prostate cancer, along with its effect on the inhibition of maspin was also found. Therefore, specific targeting IL-6TS in prostate cancer patients, might represent an interesting way to refine the currently available experimental anti-IL-6 therapies since sIL-6R and IL-6 are altered in patients with a worse prognosis. This information may be helpful to identify those patients who could benefit from the anti-cytokine therapy .
Recent studies have investigated the use of maspin as a therapeutic agent against cancer. In one study, maspin was found to inhibit cancer growth and metastasis in a breast cancer mouse model through a maspin DNA-liposome therapy. Another study showed the ability of maspin to induce apoptosis in tumor-specific endothelial cells. Taken together, these studies demonstrate the potential use of maspin as a viable anticancer therapeutic agent . Gene therapy focusing on the use of adeno-associated virus (AAV, serotype 2) vector encoding maspin in human prostate cancer was also evaluated. Immunofluorescence double staining for maspin protein and apoptosis in LNCaP tumors showed that the percentage of apoptotic cells in AAV-maspin-mediated maspin-expressing cells was significantly high if compared with that in AAV-GFP-mediated GFP-expressing cells. Moreover, significantly fewer CD31-positive microvessels were observed in AAV-maspin-treated tumors compared with the control tumors. These therapeutic responses were highly correlated to persistent maspin expression in tumors, confirmed by Western blot analysis until at least 56 days after treatment. Furthermore AAV-mediated prolonged maspin expression efficiently suppresses human prostate tumor growth in vivo by apoptosis induction and inhibition of angiogenesis .
Focusing on natural substances, it has been showed that curcumin, a hydrophobic polyphenol derived from turmeric (the rhizome of the herb Curcuma longa), is implicated in the inhibition of tumorigenesis with a combination of anti-inflammatory, anti-oxidant, immunomodulatory, pro-apoptotic, and anti-angiogenic properties via pleiotropic effects on genes and cell-signaling pathways at multiple levels, including activation of transcription factors, receptors, kinases, cytokines, enzymes and growth factors.
Curcumin has recently received a great deal of attention as a chemoprotective agent for the prevention of colon and breast cancer and it may have clinical application in the prevention of prostate cancer. Curcumin down-regulates the androgen receptor gene not only at the protein level but also in its transcriptional activity in androgen-depended prostate cancer. The suppression of androgen receptor transaction may affect the androgen receptor regulated genes such as maspin and curcumin increase the expression of maspin with dose and time dependence.
The mechanism utilized by curcumin to up-regulate maspin expression is unknown at this time. Nevertheless, the up-regulation only occurs in cells that have wild-type p53. This suggests that the activity of curcumin toward maspin expression may be modulated through the p53 pathway. Two other examples of nutritional compounds that have been reported to enhance tumor suppression and inhibit metastasis through up-regulation of maspin are abalone visceral extract and apple peel extract.
Also resveratrol (trans-3, 49, 5-trihydroxystilbene), a polyphenolic antioxidant found in peanuts, grapes and red wine possesses significant health benefits. This compound has shown beneficial effects in experimental cancer models, where it suppresses the initiation, promotion and progression of tumors Recent studies have implicated activation of the apoptotic pathway as a mechanism accounting for the antitumor benefits of resveratrol. For example, resveratrol inhibits cell proliferation, induces apoptosis of human prostate carcinoma and acute lymphoblastic leukemia cells and increases maspin levels through Akt pathway [159–163].
summary of maspin expression
Tumor progression normal to cancer
Positive cytoplasm or positive nucleus
Normal tissue: positive maspin
Positive nucleus = better prognosis
Positive maspin = better/worse prognosis depending of localization and of epigenetic modification
Cancer tissue +/−
Cancer metastasis = negative maspin
Positive nucleus increased in invasive cancer
Positive maspin = better prognosis
HGPI: positive maspin
Invasive cancer = negative maspin
Positive nucleus = better prognosis
Positive maspin = better prognosis
Negative maspin (T2 → T4 positive?)
Normal tissue: positive maspin
Positive nucleus = better prognosis vs nuclear and positive cytoplasm
Positive maspin = better prognosis
Lung cancer = less positive maspin
Normal tissue: Negative maspin
Positive nucleus (rare) = better prognosis
Positive maspin = better/worse prognosis depending of localization and of epigenetic modification
Ovarian cancer = positive maspin
Metastic cancer = less positive
Normal tissue: negative maspin
Positive nucleus = worse prognosis
Positive maspin = better prognosis, positive response to 5FU therapy
Dysplasia and cancer = positive maspin
Positive cytoplasm better prognosis
Normal tissue: positive maspin
Positive nucleus and positive cytoplasm = better prognosis vs only positive cytoplasm
Positive maspin = better prognosis
Cancer tissue: negative maspin
Normal tissue: negative maspin
Positive maspin = worse prognosis
Pan In1, Pan In2 = negative maspin
Pan In3 and cancer invasive tissue = positive maspin
Normal tissue: dysplasia = negative maspin intestinal metastasia and cancer = positive maspin
Head and neck cancer
Positive nucleus = better prognosis
Positive maspin = better prognosis
Normal tissue adenomas and follicular carcinoma = negative maspin/+ papillary carcinoma = positive maspin
Positive maspin = better prognosis
Anaplastic and poorly differentiated = negative maspin
Normal tissue: positive maspin
Positive nucleus = better prognosis
Positive maspin = better prognosis
Melanoma = positive maspin
Metastatic melanoma = negative maspin
The correlation between the expression of maspin and clinical parameters is different depending on the type of tumor. It is likely that the lack of standardization in assessing the positivity of maspin subcellular level and the need for more studies about it can explain and permit the interpretation of differences. It would be more helpful to evaluate the expression of maspin only as a positive nucleus-cytoplasm to ensure greater uniformity of results.
- Zou Z, Anisowicz A, Hendrix MJ, Thor A, Neveu M, Sheng S, Rafidi K, Seftor E, Sager R: Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science 1994, 263(5146):526–529. 10.1126/science.8290962PubMedView ArticleGoogle Scholar
- Klasa-Mazurkiewicz D, Narkiewicz J, Milczek T, Lipińska B, Emerich J: Maspin overexpression correlates with positive response to primary chemotherapy in ovarian cancer patients. Gynecol Oncol 2009, 113(1):91–98. Epub 2009 Feb 3 Epub 2009 Feb 3 10.1016/j.ygyno.2008.12.038PubMedView ArticleGoogle Scholar
- Lockett J, Yin S, Li X, Meng Y, Sheng S: Tumor suppressive maspin and epithelial homeostasis. J Cell Biochem 2006, 97(4):651–660. 10.1002/jcb.20721PubMedView ArticleGoogle Scholar
- Chen EI, Florens L, Axelrod FT, Monosov E, Barbas CF 3rd, Yates JR 3rd, Felding-Habermann B, Smith JW: Maspin alters the carcinoma proteome. FASEB J 2005, 19(9):1123–1124. Epub 2005 Apr 27 Epub 2005 Apr 27PubMedGoogle Scholar
- Law RH, Irving JA, Buckle AM, Ruzyla K, Buzza M, Bashtannyk-Puhalovich TA, Beddoe TC, Nguyen K, Worrall DM, Bottomley SP, Bird PI, Rossjohn J, Whisstock JC: The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix. J Biol Chem 2005, 280(23):22356–22364. 10.1074/jbc.M412043200PubMedView ArticleGoogle Scholar
- Beecken WD, Kramer W, Jonas D: New molecular mediators in tumor angiogenesis. J Cell Mol Med 2000, 4(4):262–269. 10.1111/j.1582-4934.2000.tb00125.xPubMedView ArticleGoogle Scholar
- Pemberton PA, Wong DT, Gibson HL, Kiefer MC, Fitzpatrick PA, Sager R, Barr PJ: The tumor suppressor maspin does not undergo the stressed to relaxed transition or inhibit trypsin-like serine proteases evidence that maspin is not a protease inhibitory serpin. ASBMB 1995, 270(26):15832–15837.Google Scholar
- D’Andrea LD, Del Gatto A, Pedone C, Benedetti E: Peptide-based molecules in angiogenesis. Chem Biol Drug Des 2006, 67(2):115–126. 10.1111/j.1747-0285.2006.00356.xPubMedView ArticleGoogle Scholar
- Vogt GR: Sticky Situations: A Story of the Rebel Mammary Serine Protease Inhibitor. Brown Deer High School Poster 2007–2008 Project: Center for bioMolecular Modeling;
- Cella N, Contreras A, Latha K, Rosen JM, Zhang M: Maspin is physically associated with [beta]1 integrin regulating cell adhesion in mammary epithelial cells. FASEB J 2006, 20(9):1510–1512. Epub 2006 May 23 Epub 2006 May 23 10.1096/fj.05-5500fjePubMedView ArticleGoogle Scholar
- Ravenhill L, Wagstaff L, Edwards DR, Ellis V, Bass R: G-helix of maspin mediates effects on cell migration and adhesion. J Biol Chem 2010, 285(47):36285–36292. 10.1074/jbc.M110.177253PubMed CentralPubMedView ArticleGoogle Scholar
- Seftor RE, Seftor EA, Sheng S, Pemberton PA, Sager R, Hendrix MJ: Maspin suppresses the invasive phenotype of human breast carcinoma. Cancer Res 1998, 58(24):5681–5685.PubMedGoogle Scholar
- Zhang W, Shi HY, Zhang M: Maspin overexpression modulates tumor cell apoptosis through the regulation of Bcl-2 family proteins. BMC Cancer 2005, 5: 50. 10.1186/1471-2407-5-50PubMed CentralPubMedView ArticleGoogle Scholar
- Shi HY, Zhang W, Liang R, Kittrell F, Templeton NS, Medina D, Zhang M: Modeling human breast cancer metastasis in mice: maspin as a paradigm. Histol Histopathol 2003, 18: 201–206.PubMedGoogle Scholar
- Affara NI, Coussens LM: IKKα at the crossroads of inflammation and metastasis. Cell 2007, 129(1):25–26. 10.1016/j.cell.2007.03.029PubMedView ArticleGoogle Scholar
- Sheng S: Maspin: a novel serine protease inhibitor. Canc Growth Progression 2005, 15: 407–418. 10.1007/1-4020-3414-8_23View ArticleGoogle Scholar
- Jang HL, Nam E, Lee KH, Yeom S, Son HJ, Park C: Maspin polymorphism associated with apoptosis susceptibility and in vivo tumorigenesis. Int J Mol Med 2008, 22(3):333–338.PubMedGoogle Scholar
- Chen EI, Yates JR: Maspin and tumor metastasis. UBMB Life. 2006, 58(1):25–29.View ArticleGoogle Scholar
- Goulet B, Chan G, Chambers AF, Lewis JD: An emerging role for the nuclear localization of maspin in the suppression of tumr progression and metastasis. Biochem Cell Biol 2012, 90(1):22–38. Epub 2011 Nov 2 Epub 2011 Nov 2PubMedGoogle Scholar
- Zhang M, Magit D, Botteri F: Maspin plays an important role in mammary gland development. Dev Biol 1999, 215: 278–287. 10.1006/dbio.1999.9442PubMedView ArticleGoogle Scholar
- Pemberton PA, Tipton AR, Pavloff N: Maspin is an intracellular serpin that partitions into secretory vesicles and is present at the cell surface. J Histochem Cytochem 1997, 45: 1697–70613. 10.1177/002215549704501213PubMedView ArticleGoogle Scholar
- Khalkhali-Ellis Z: Maspin: the new frontier. Clin Cancer Res 2006, 12: 7279–7283. 10.1158/1078-0432.CCR-06-1589PubMed CentralPubMedView ArticleGoogle Scholar
- Blacque OE, Worrall DM: Evidence for a direct interaction between the tumor suppressor serpin, maspin, and types I and III collagen. J Biol Chem 2002, 277: 10783. 10.1074/jbc.M110992200PubMedView ArticleGoogle Scholar
- Khalkhali-Ellis Z, Hendrix MJ: Elucidating the function of secreted maspin: inhibiting cathepsin D-mediated matrix degradation. Cancer Res 2007, 67(8):3535–3539. 10.1158/0008-5472.CAN-06-4767PubMed CentralPubMedView ArticleGoogle Scholar
- Goulet B, Kennette W, Ablack A, Postenka CO, Hague MN, Mymryk JS, Tuck AB, Giguère V, Chambers AF, Lewis JD: Nuclear localization of maspin is essential for its inhibition of tumor growth and metastasis. Lab Invest 2011, 91(8):1181–1187. Epub 2011 Apr 18 Epub 2011 Apr 18 10.1038/labinvest.2011.66PubMedView ArticleGoogle Scholar
- Teoh SS, Whisstock JC, Bird PI: Maspin (SERPINB5) is an obligate intracellular serpin. J Biol Chem 2010, 285(14):10862–10869. Epub 2010 Feb 1 Epub 2010 Feb 1 10.1074/jbc.M109.073171PubMed CentralPubMedView ArticleGoogle Scholar
- Sood AK, Fletcher MS, Gruman LM, Coffin JE, Jabbari S, Khalkhali-Ellis Z, Arbour N, Seftor EA, Hendrix MJ: The paradoxical expression of maspin in ovarian carcinoma. Clin Cancer Res 2002, 8(9):2924–2932.PubMedGoogle Scholar
- Beltran AS, Blancafort P: Reactivation of MASPIN in non-small cell lung carcinoma (NSCLC) cells by artificial transcription factors (ATFs). Epigenetics 2011, 6(2):224–235. Epub 2011 Feb 1 Epub 2011 Feb 1 10.4161/epi.6.2.13700PubMed CentralPubMedView ArticleGoogle Scholar
- Wu Y, Alvarez M, Slamon DJ, Koeffler P, Vadgama JV: Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation. BMC Cancer 2010, 10: 32. 10.1186/1471-2407-10-32PubMed CentralPubMedView ArticleGoogle Scholar
- Bodenstine TM, Seftor REB, Khalkhali-Ellis Z, Seftor EA, Pemberton PA, Hendrix MJC: Maspin: molecular mechanisms and therapeutic implications. Cancer Metastasis Rev December 2012, 31(3–4):529–551.View ArticleGoogle Scholar
- Bettstetter M, Woenckhaus M, Wild PJ, Rümmele P, Blaszyk H, Hartmann A, Hofstädter F, Dietmaier W: Elevated nuclear maspin expression is associated with microsatellite instability and high tumour grade in colorectal cancer. J Pathol 2005, 205(5):606–614. 10.1002/path.1732PubMedView ArticleGoogle Scholar
- Latha K, Zhang W, Cella N, Shi HY, Zhang M: Maspin mediates increased tumor cell apoptosis upon induction of the mitochondrial permeability transition. Mol Cell Biol 2005, 25: 1737–1748. 10.1128/MCB.25.5.1737-1748.2005PubMed CentralPubMedView ArticleGoogle Scholar
- Liu J, Yin S, Reddy N, Spencer C, Sheng S: Bax mediates the apoptosis-sensitizing effect of maspin. Cancer Res 2004, 64: 1703–1711. 10.1158/0008-5472.CAN-03-2568PubMedView ArticleGoogle Scholar
- Sheng S: A role of novel serpin maspin in tumor progression: the divergence revealed through efforts to converge. J Cell Physiol 2006, 209: 631–635. 10.1002/jcp.20786PubMedView ArticleGoogle Scholar
- Biliran H Jr, Sheng S: Pleiotropic inhibition of pericellular urokinase-type plasminogen activator system by endogenous tumor suppressive maspin. Cancer Res 2001, 61: 8676–8682.PubMedGoogle Scholar
- Amir S, Margaryan NV, Odero-Marah V, Khalkhali-Ellis Z, Hendrix MJC: Maspin regulates hypoxia-mediated stimulation of uPA/uPAR complex in invasive breast cancer cells. Cancer Biol Ther 2005, 4: 400–406. 10.4161/cbt.4.4.1617PubMed CentralPubMedView ArticleGoogle Scholar
- Yin S, Lockett J, Meng Y, Biliran HJ, Blouse GE, Li X, Reddy N, Zhao Z, Lin X, Anagli J, Cher ML, Sheng S: Maspin retards cell detachment via a novel interaction with the urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor system. Cancer Res 2006, 66: 4173–4181. 10.1158/0008-5472.CAN-05-3514PubMedView ArticleGoogle Scholar
- Ossowski L, Aguirre-Ghiso JA: Urokinase receptor and integrin partnership: coordination of signaling for cell adhesion, migration and growth. Curr Opin Cell Biol 2000, 12: 613–620. 10.1016/S0955-0674(00)00140-XPubMedView ArticleGoogle Scholar
- Odero-Marah VA, Khalkhali-Ellis Z, Chunthapong J, Amir S, Seftor RE, Seftor EA, Hendrix MJC: Maspin regulates different signaling pathways for motility and adhesion in aggressive breast cancer cells. Cancer Biol Ther 2003, 2: 398–403.PubMedView ArticleGoogle Scholar
- Toillon RA, Lagadec C, Page A, Chopin V, Sautière PE, Ricort JM, Lemoine J, Zhang M, Hondermarck H, Le Bourhis X: Proteomics demonstration that normal breast epithelial cells can induce apoptosis of breast cancer cells through insulin-like growth factor-binding protein-3 and maspin. Mol Cell Proteomics 2007, 6(7):1239–1247. Epub 2007 Apr 19 Epub 2007 Apr 19 10.1074/mcp.M600477-MCP200PubMedView ArticleGoogle Scholar
- Maass N, Teffner M, Rösel F, Pawaresch R, Jonat W, Nagasaki K, Rudolph P: Decline in the expression of the serine proteinase inhibitor maspin is associated with tumour progression in ductal carcinomas of the breast. J Pathol 2001, 195(3):321–326. 10.1002/path.948PubMedView ArticleGoogle Scholar
- Joensuu KM, Leidenius MH, Andersson LC, Heikkilä PS: High expression of maspin is associated with early tumor relapse in breast cancer. Hum Pathol 2009, 40(8):1143–1151. Epub 2009 May 8 Epub 2009 May 8 10.1016/j.humpath.2009.02.006PubMedView ArticleGoogle Scholar
- Sharma G, Mirza S, Parshad R, Srivastava A, Gupta SD, Pandya P, Ralhan R: Clinical significance of Maspin promoter methylation and loss of its protein expression in invasive ductal breast carcinoma: correlation with VEGF-A and MTA1 expression. Tumour Biol 2011, 32(1):23–32. Epub 2010 Epub 2010 10.1007/s13277-010-0087-8PubMedView ArticleGoogle Scholar
- Hojo T, Akiyama Y, Nagasaki K, Maruyama K, Kikuchi K, Ikeda T, Kitajima M, Yamaguchi K: Association of maspin expression with the malignancy grade and tumor vascularization in breast cancer tissues. Cancer Lett 2001, 171: 103–110. 10.1016/S0304-3835(01)00569-9PubMedView ArticleGoogle Scholar
- Xia W, Lau YK, Hu MC, Li L, Johnston DA, Sheng S, El-Naggar A, Hung MC: High tumoral maspin expression is associated with improved survival of patients with oral squamous cell carcinoma. Oncogene 2000, 19: 2398. 10.1038/sj.onc.1203535PubMedView ArticleGoogle Scholar
- Umekita Y, Yoshida H: Expression of maspin is up-regulated during the progression of mammary ductal carcinoma. Histopathology 2003, 42(6):541–545. 10.1046/j.1365-2559.2003.01620.xPubMedView ArticleGoogle Scholar
- Kim DH, Yoon DS, Dooley WC, Nam ES, Ryu JW, Jung KC, Park HR, Sohn JH, Shin HS, Park YE: Association of maspin expression with the high histological grade and lymphocyte-rich stroma in early-stage breast cancer. Histopathology 2003, 42(1):37–42. 10.1046/j.1365-2559.2003.01567.xPubMedView ArticleGoogle Scholar
- Mohsin SK, Zhang M, Clark GM, Craig Allred D: Maspin expression in invasive breast cancer: association with other prognostic factors. J Pathol 2003, 199(4):432–435. 10.1002/path.1319PubMedView ArticleGoogle Scholar
- Umekita Y, Ohi Y, Sagara Y, Yoshida H: Expression of maspin predicts poor prognosis in breast-cancer patients. Int J Cancer 2002, 100(4):452–455. 10.1002/ijc.10500PubMedView ArticleGoogle Scholar
- Umekita Y, Ohi Y, Souda M, Rai Y, Sagara Y, Sagara Y, Tamada S, Tanimoto A: Maspin expression is frequent and correlates with basal markers in triple-negative breast cancer. Diagn Pathol 2011, 6: 36. 10.1186/1746-1596-6-36PubMed CentralPubMedView ArticleGoogle Scholar
- Lee MJ, Suh CH, Li ZH: Clinicopathological Significance of Maspin Expression in Breast Cancer. J Korean Med Sci 2006, 21(2):309–314. 10.3346/jkms.2006.21.2.309PubMed CentralPubMedView ArticleGoogle Scholar
- Stark AM, Schem C, Maass N, Hugo HH, Jonat W, Mehdorn HM, Held-Feindt J: Expression of metastasis suppressor gene maspin is reduced in breast cancer brain metastases and correlates with the estrogen receptor status. Neurol Res 2010, 32(3):303–308. Epub 2009 Nov 11 Epub 2009 Nov 11 10.1179/016164109X12518779082192PubMedView ArticleGoogle Scholar
- Maass N, Hojo T, Rösel F, Ikeda T, Jonat W, Nagasaki K: Down regulation of the tumor suppressor gene maspin in breast carcinoma is associated with a higher risk of distant metastasis. Clin Biochem 2001, 34(4):303–307. 10.1016/S0009-9120(01)00220-XPubMedView ArticleGoogle Scholar
- Pierson CR, McGowen R, Grignon D, Sakr W, Dey J, Sheng S: Maspin is up-regulated in premalignant prostate epithelia. Prostate 2002, 53(4):255–262. 10.1002/pros.10107PubMedView ArticleGoogle Scholar
- Blacque OE, Worrall DM: J: Evidence for a direct interaction between the Tumor Suppressor Serpin, Maspin, and Types I and III Collagen. Biol Chem 2002, 277: 10783–10788. 10.1074/jbc.M110992200View ArticleGoogle Scholar
- Cher ML, Biliran HR, Bhagat S, Meng Y, Che M, Lockett J, Abrams J, Fridman R, Zachareas M, Sheng S, Biliran H Jr, Sheng S: Maspin expression inhibits osteolysis, tumor growth, and angiogenesis in a model of prostate cancer bone metastasis. 2003, 100(13):7847–7852.Google Scholar
- Bernardo MM, Meng Y, Lockett J, Dyson G, Dombkowski A, Kaplun A, Li X, Yin S, Dzinic S, Olive M, Dean I, Krass D, Moin K, Bonfil RD, Cher M, Sakr W, Sheng S: Maspin reprograms the gene expression profile of prostate carcinoma cells for differentiation. Genes Cancer 2011, 2(11):1009–1022. 10.1177/1947601912440170PubMed CentralPubMedView ArticleGoogle Scholar
- Neal CL, Henderson V, Smith BN, McKeithen D, Graham T, Baohan T, Odero-Marah VA: Snail transcription factor negatively regulates maspin tumor suppressor in human prostate cancer cells. BMC Cancer 2012, 12: 336. 10.1186/1471-2407-12-336PubMed CentralPubMedView ArticleGoogle Scholar
- McKenzie S, Sakamoto S, Kyprianou N: Maspin modulates prostate cancer cell apoptotic and angiogenic response to hypoxia via targeting AKT. Oncogene 2008, 27(57):7171–7179. Published online 2008 October 20 Published online 2008 October 20 10.1038/onc.2008.321PubMed CentralPubMedView ArticleGoogle Scholar
- Zhang M, Magit D, Sager R: Expression of maspin in prostate cells is regulated by a positive Ets element and a negative hormonal responsive element site recognized byandrogenreceptor. Cell Biology 1997, 94(11):5673–5678.Google Scholar
- Tahmatzopoulos A, Sheng S, Kyprianou N: Maspin sensitizes prostate cancer cells to doxazosin-induced apoptosis. Oncogene 2005, 24(34):5375–5383. 10.1038/sj.onc.1208684PubMed CentralPubMedView ArticleGoogle Scholar
- Lovrić E, Gatalica Z, Eyzaguirre E, Kruslin B: Expression of maspin and glutathionine-S-transferase-pi in normal human prostate and prostatic carcinomas. Appl Immunohistochem Mol Morphol 2010, 18(5):429–432.PubMedGoogle Scholar
- Riddick ACP, Shukla CJ, Pennington CJ, Bass R, Nuttall RK, Hogan A, Sethia KK, Ellis V, Collins AT, Maitland NJ, Ball RY, Edwards DR: Identification of degradome components associated with prostate cancer progression by expression analysis of human prostatic tissues. Br J Cancer 2005, 92(12):2171–2180. 10.1038/sj.bjc.6602630PubMed CentralPubMedView ArticleGoogle Scholar
- Machtens S, Serth J, Bokemeyer C, Bathke W, Minssen A, Kollmannsberger C, Hartmann J, Knüchel R, Kondo M, Jonas U, Kuczyk M: Expression of the p53 and maspin protein in primary prostate cancer: Correlation with clinical features. Int J Cancer 2001, 95: 337–342. 10.1002/1097-0215(20010920)95:5<337::AID-IJC1059>3.0.CO;2-1PubMedView ArticleGoogle Scholar
- Zou Z, Zhang W, Young D, Gleave MG, Rennie P, Connell T, Connelly R, Moul J, Srivastava S, Sesterhenn I: Maspin expression profile in human prostate cancer (CaP) and in vitro induction of Maspin expression by androgen ablation. Clin Cancer Res 2002, 8(5):1172–1177.PubMedGoogle Scholar
- Blacque OE, Worrall DM: Evidence for a direct interaction between the tumor suppressor serpin, maspin, and types I and II collagen. J Biol Chem 2002, 27: 10783–10788.View ArticleGoogle Scholar
- Ngamkitidechakul C, Warejcka DJ, Burke JM, O’Brien WJ, Twining SS: Sufficiency of the reactive site loop of maspin for induction of cell matrix adhesion and inhibition of cell invasion. J Biol Chem 2003, 278: 31796–31806. 10.1074/jbc.M302408200PubMedView ArticleGoogle Scholar
- Bass R, Moreno Fernandez A-M, Ellis V: Maspin inhibits cell migration in the absence of protease inhibitory activity. J Biol Chem 2002, 277: 46845–46848. 10.1074/jbc.C200532200PubMedView ArticleGoogle Scholar
- Oshiro MM, Watts GS, Wozniak RJ, Junk DJ, Munoz-Rodriguez JL, Domann FE, Futscher BW: Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression. Oncogene 2003, 22: 3624–3634. 10.1038/sj.onc.1206545PubMedView ArticleGoogle Scholar
- Zhang W, Zhang M: Tissue microarray analysis of maspin expression and its reverse correlation with mutant p53 in various tumors. Int J Oncol 2002, 20: 1145–1150.PubMedGoogle Scholar
- Machtens S, Serth J, Bokemeyer C, Bathke W, Minssen A, Kollmannsberger C, Hartmann J, Knuchel R, Kondo M, Jonas U, Kuczyk M: Expression of p53 and maspin protein in primary prostate cancer: correlation with clinical features. Int J Cancer 2001, 95: 337–342. 10.1002/1097-0215(20010920)95:5<337::AID-IJC1059>3.0.CO;2-1PubMedView ArticleGoogle Scholar
- Marioni G, Blandamura S, Giacomelli L, Calgaro N, Segato P, Leo G, Fischetto D, Staffieri A, de Filippis C: Nuclear expression of maspin is associated with a lower recurrence rate and a longer disease-free interval after surgery for squamous cell carcinoma of the larynx. Histopathology 2005, 46: 576–582. 10.1111/j.1365-2559.2005.02141.xPubMedView ArticleGoogle Scholar
- Beecken WD, Engl T, Engels K, Blumenberg C, Oppermann E, Camphausen K, Shing Y, Reinecke G, Jonas D, Blaheta R: Clinical relevance of maspin expression in bladder cancer. World J Urol 2006, 24: 338–344. 10.1007/s00345-006-0085-zPubMedView ArticleGoogle Scholar
- Montironi R, Lopez-Beltran A: The 2004 WHO classification of bladder tumors: a summary and commentary. Int J Surg Pathol 2005, 13: 143–153. 10.1177/106689690501300203PubMedView ArticleGoogle Scholar
- Oosterlink W: Guidelines on diagnosis and treatment of superficial bladder cancer. Minerva Urol Nefrol 2004, 56: 65–72.Google Scholar
- Li Z, Shi HY, Zhang M: Targeted expression of maspin in tumor vasculatures induces endothelial cell apoptosis. Oncogene 2005, 24: 2008–2019. 10.1038/sj.onc.1208449PubMedView ArticleGoogle Scholar
- Pemberton PA, Tipton AR, Pavloff N, Smith J, Erickson JR, Mouchabeck ZM, Kiefer MC: Maspin is an intracellular serpin that partitions into secretory vesicles and is present at the cell surface. J Histochem Cytochem 1997, 45: 1697–1706. 10.1177/002215549704501213PubMedView ArticleGoogle Scholar
- McGowen R, Biliran H Jr, Sager R, Sheng S: The surface of prostate carcinoma DU145 cells mediates the inhibition of urokinase-type plasminogen activator by maspin. Cancer Res 2000, 60: 4771–4778.PubMedGoogle Scholar
- Woenckhaus M, Bubendorf L, Dalquen P, Foerster J, Blaszyk H, Mirlacher M, Soler M, Dietmaier W, Sauter G, Hartmann A, Wild PJ: Nuclear and cytoplasmic maspin expression in primary non-small cell lung cancer. J Clin Pathol 2007, 60: 483–486.PubMed CentralPubMedView ArticleGoogle Scholar
- Acikalin D, Oner U, Can C, Acikalin MF, Ertugrul Colak E: Predictive value of maspin and Ki-67 expression in transurethral resection specimens in patients with T1 bladder cancer. Tumori 2012, 98(3):344–350.PubMedGoogle Scholar
- Kramer MW, Waalkes S, Hennenlotter J, Serth J, Stenzl A, Kuczyk MA, Merseburger AS: Maspin protein expression correlates with tumor progression in non-muscle invasive bladder cancer. Oncol Lett. 2010, 1(4):621–626.PubMed CentralPubMedGoogle Scholar
- Friedrich MG, Toma MI, Petri S, Cheng JC, Hammerer P, Erbersdobler A, Huland H: Expression of Maspin in non-muscle invasive bladder carcinoma: correlation with tumor angiogenesis and prognosis. Eur Urol 2004, 45(6):737–743. 10.1016/j.eururo.2003.12.005PubMedView ArticleGoogle Scholar
- Sugimoto S, Maass N, Takimoto Y, Sato K, Minei S, Zhang M, Hoshikawa Y, Jünemann KP, Jonat W, Nagasaki K: Expression and regulation of tumor suppressor gene maspin in human bladder cancer. Cancer Lett 2004, 203(2):209–215. 10.1016/j.canlet.2003.09.010PubMedView ArticleGoogle Scholar
- Nehad MR, Abd El-Maqsoud MD, Ehab Rifat Tawfiek MD: Loss of maspin expression in bladder cancer: its relationship with p53 and clinicopathological parameters. Journal of the Egyptian Nat Cancer Inst. 2010, 22(1):1–12.Google Scholar
- Blandamura S, D’Alessandro E, Giacomelli L, Guzzardo V, Battanello W, Repele M, Ninfo V: Expression of maspin in papillary Ta/T1 bladder neoplasms. Anticancer Res 2008, 28(1B):471–478.PubMedGoogle Scholar
- Nam E, Park C: Maspin suppresses survival of lung cancer cells through modulation of Akt pathway. Cancer Res Trea 2010, 42(1):42–47. Epub 2010 Mar 31 Epub 2010 Mar 31 10.4143/crt.2010.42.1.42View ArticleGoogle Scholar
- Bircan A, Bircan S, Kapucuoglu N, Songur N, Ozturk O, Akkaya A: Maspin: VEGF and p53 expression in small biopsies of primary advanced lung cancer and relationship with clinicopathologic parameters. Pathol Oncol Res 2010, 16(4):553–561. 10.1007/s12253-010-9259-5PubMedView ArticleGoogle Scholar
- Lonardo F, Li X, Siddiq F, Singh R, Al-Abbadi M, Pass HI, Sheng S: Maspin nuclear localization is linked to favorable morphological features in pulmonary adenocarcinoma. Lung Cancer 2006, 51(1):31–39. Epub 2005 Sep 12 Epub 2005 Sep 12 10.1016/j.lungcan.2005.07.011PubMedView ArticleGoogle Scholar
- Frey A, Soubani AO, Adam AK, Sheng S, Pass HI, Lonardo F: Nuclear, compared with combined nuclear and cytoplasmic expression of maspin, is linked in lung adenocarcinoma to reduced VEGF-A levels and in Stage I, improved survival. Histopathology 2009, 54(5):590–597. Epub 2009 Mar 20 Epub 2009 Mar 20 10.1111/j.1365-2559.2009.03260.xPubMed CentralPubMedView ArticleGoogle Scholar
- Hirai K, Koizumi K, Haraguchi S, Hirata T, Mikami I, Fukushima M, Yamagishi S, Kawashima T, Okada D, Shimizu K, Kawamoto M: Prognostic significance of the tumor suppressor gene maspin in non-small cell lung cancer. Ann Thorac Surg 2005, 79(1):248–253. 10.1016/j.athoracsur.2004.06.118PubMedView ArticleGoogle Scholar
- Berardi R, Santinelli A, Onofri A, Brunelli A, Pierantoni C, Pisa E, Pagliacci A, Stramazzotti D, Zuccatosta L, Mazzanti P, Sabbatini A, Gasparini S, Bearzi I, Cascinu S: Maspin expression is a favorable prognostic factor in non-small cell lung cancer. Anal Quant Cytol Histol 2012, 34(2):72–78.PubMedGoogle Scholar
- Wu S, Yu L, Cheng Z, Song W, Zhou L, Tao Y: Expression of maspin in non-small cell lung cancer and its relationship to vasculogenic mimicry. J Huazhong Univ Sci Technolog Med Sci 2012, 32(3):346–352. Epub 2012 Jun 9 Epub 2012 Jun 9 10.1007/s11596-012-0060-4PubMedView ArticleGoogle Scholar
- Nakagawa M, Katakura H, Adachi M, Takenaka K, Yanagihara K, Otake Y, Wada H, Tanaka F: Maspin expression and its clinical significance in non-small cell lung cancer. Ann Surg Oncol 2006, 13(11):1517–1523. 10.1245/s10434-006-9030-zPubMedView ArticleGoogle Scholar
- Katakura H, Takenaka K, Nakagawa M, Sonobe M, Adachi M, Ito S, Wada H, Tanaka F: Maspin gene expression is a significant prognostic factor in resected non-small cell lung cancer (NSCLC). Maspin in NSCLC. Lung Cancer 2006, 51(3):323–328. Epub 2006 Jan 6 Epub 2006 Jan 6 10.1016/j.lungcan.2005.10.017PubMedView ArticleGoogle Scholar
- Takanami I, Abiko T, Koizumi S: Expression of maspin in non-small-cell lung cancer: correlation with clinical features. Clin Lung Cancer 2008, 9(6):361–366. 10.3816/CLC.2008.n.052PubMedView ArticleGoogle Scholar
- Lin Z, Liu Y, Sun Y, He X: Expression of Ets-1, Ang-2 and maspin in ovarian cancer and their role in tumor angiogenesis. Clin Cancer Res 2002, 8(9):2924–2932. Journal of Experimental & Clinical Cancer Research 2011, 30:31 Journal of Experimental & Clinical Cancer Research 2011, 30:31Google Scholar
- Secord AA, Lee PS, Darcy KM, Havrilesky LJ, Grace LA, Marks JR, Berchuck A, Gynecologic oncology group: Maspin expression in epithelial ovarian cancer and associations with poor prognosis: a gynecologic oncology group study. Gynecol Oncol 2006, 101: 390–397. 10.1016/j.ygyno.2006.02.014PubMedView ArticleGoogle Scholar
- Moshira Mohamad Abd El Wahed MD: Expression and Subcellular Localization of Maspin in Human Ovarian Epithelial Neoplasms: Correlation with Clinicopathologic Features. Journal of the Egyptian Nat. Cancer Inst 2005, 17(3):173–183.PubMedGoogle Scholar
- Sopel M, Surowiak P, Berdowska I: Nuclear maspin expression as a good prognostic factor in human epithelial ovarian carcinoma. Folia Morphol (Warsz) 2010, 69(4):204–212.Google Scholar
- Solomon LA, Munkarah AR, Schimp VL, Arabi MH, Morris RT, Nassar H, Ali-Fehmi R: Maspinexpression and localization impact on angiogenesis and prognosis in ovarian cancer. Gynecol Oncol 2006, 101(3):385–389. 10.1016/j.ygyno.2005.11.049PubMedView ArticleGoogle Scholar
- Bolat F, Gumurdulu D, Erkanli S, Kayaselcuk F, Zeren H, Ali Vardar M, Kuscu E: Maspin overexpression correlates with increased expression of vascular endothelial growth factors A, C, and D in human ovarian carcinoma. Pathol Res Pract 2008, 204(6):379–387. Epub 2008 Mar 17 Epub 2008 Mar 17 10.1016/j.prp.2008.01.011PubMedView ArticleGoogle Scholar
- Bauerschlag DO, Habermann M, Weimer J, Meinhold-Heerlein I, Hilpert F, Weigel M, Bauer M, Mundhenke C, Jonat W, Maass N, Schem C: Heterogeneous expression of serine protease inhibitor maspin in ovarian cancer. Anticancer Res 2010, 30(7):2739–2744.PubMedGoogle Scholar
- Cao D, Wilentz RE, Abbruzzese JL, Ho L, Maitra A: Aberrant expression of maspin in idiopathic inflammatory bowel disease is associated with disease activity and neoplastic transformation. Int J Gastrointest Cancer 2005, 36(1):39–46. 10.1385/IJGC:36:1:039PubMedView ArticleGoogle Scholar
- Song SY, Lee SK, Kim DH, Son HJ, Kim HJ, Lim YJ, Lee WY, Chun HK, Rhee JC: Expression of maspin in colon cancers: its relationship with p53 expression and microvessel density. Dig Dis Sci 2002, 47(8):1831–1835. 10.1023/A:1016409031562PubMedView ArticleGoogle Scholar
- Ni JT, Yi YF, Shi HP: Expressions of maspin, P53 and Skp2 in colorectal tumors and their clinicopathological significance. Chin J Canc Res 2009, 21(2):147–153. 10.1007/s11670-009-0147-zView ArticleGoogle Scholar
- Zheng H, Tsuneyama K, Cheng C, Takahashi H, Cui Z, Murai Y, Nomoto K, Takano Y: Maspin expression was involved in colorectal adenoma-adenocarcinoma sequence and liver metastasis of tumors. Anticancer Res 2007, 27: 259–266.PubMedGoogle Scholar
- Umekita Y, Souda M, Yoshida H: Expression of maspin in colorectal cancer. In Vivo 2006, 20(6B):797–800.PubMedGoogle Scholar
- Fung CL-S, Chan C, Jankova L, Dent OF, Robertson G, Molloy M, Bokey L, Chapuis PH, Lin BPC, Clarke SJ: Clinicopathological correlates and prognostic significance of maspin expression in 450 patients after potentially curative resection of node-positive colonic cancer. Histopathology 2010, 56: 319–330. 10.1111/j.1365-2559.2010.03479.xPubMedView ArticleGoogle Scholar
- Gurzu S, Szentirmay Z, Toth E, Jung I: Possible predictive value of maspin expression in colorectal cancer. Recent Pat Anticancer Drug Discov 2012. PMID 22963136 PMID 22963136Google Scholar
- Märkl B, Arnholdt HM, Jähnig H, Schenkirsch G, Herrmann RA, Haude K, Spatz H, Anthuber M, Schlimok G, Oruzio D: Shift from cytoplasmic to nuclear maspin expression correlates with shorter overall survival in node-negative colorectal cancer. Hum Pathol 2010, 41(7):1024–1033. Epub 2010 Mar 23 Epub 2010 Mar 23 10.1016/j.humpath.2009.10.021PubMedView ArticleGoogle Scholar
- Dietmaier W, Bettstetter M, Wild PJ, Woenckhaus M, Rümmele P, Hartmann A, Dechant S, Blaszyk H, Pauer A, Klinkhammer-Schalke M, Hofstädter F: Nuclear Maspin expression is associated with response to adjuvant 5-fluorouracil based chemotherapy in patients with stage III colon cancer. Int J Cancer 2006, 118(9):2247–54. 10.1002/ijc.21620PubMedView ArticleGoogle Scholar
- Kim M, Ju H, Lim B, Kang C: Maspin genetically and functionally associates with gastric cancer by regulating cell cycle progression. Carcinogenesis 2012, 33(12):2344–50. 10.1093/carcin/bgs280PubMedView ArticleGoogle Scholar
- Wang MC, Yang YM, Li XH, Dong F, Li Y: Maspin expression and its clinicopathological significance in tumorigenesis and progression of gastric cancer. World J Gastroenterol 2004, 10(5):634–7.PubMedView ArticleGoogle Scholar
- Lee DY, Park CS, Kim HS, Kim JY, Kim YC, Lee S: Maspin and p53 protein expression in gastric adenocarcinoma and its clinical applications. Appl Immunohistochem Mol Morphol 2008, 16(1):13–8.PubMedGoogle Scholar
- Liu H, Shi J, Anandan V, Wang HL, Diehl D, Blansfield J, Gerhard G, Lin F: Reevaluation and identification of the best immunohistochemical panel (pVHL, Maspin, S100P, IMP-3) for ductal adenocarcinoma of the pancreas. Arch Pathol Lab Med 2012, 136(6):601–9. 10.5858/arpa.2011-0326-OAPubMedView ArticleGoogle Scholar
- Maass N, Hojo T, Ueding M, Lüttges J, Klöppel G, Jonat W, Nagasaki K: Expression of the tumor suppressor gene Maspin in human pancreatic cancers. Clin Cancer Res 2001, 7(4):812–7.PubMedGoogle Scholar
- Maesawa C, Ogasawara S, Yashima-Abo A, Kimura T, Kotani K, Masuda S, Nagata Y, Iwaya T, Suzuki K, Oyake T, Akiyama Y, Kawamura H, Masuda T: Aberrant maspin expression in gallbladder epithelium is associated with intestinal metaplasia in patients with cholelithiasis. J Clin Pathol 2006, 59: 328–330. 10.1136/jcp.2005.027284PubMed CentralPubMedView ArticleGoogle Scholar
- Kim J, Jang KT, Hyun Kim K, Wook Park J, Jin Chang B, Hyuck Lee K, Kyun Lee J, Seok Heo J, Choi SH, Wook Choi D: Aberrant maspin expression is involved in early carcinogenesis of gallbladder cancer. Tumor Biology 2010, 31(5):471–476. 10.1007/s13277-010-0056-2PubMedView ArticleGoogle Scholar
- Xia W, Lau YK, Hu M-T: High tumoral maspin expression is associated with improved survival of patients with oral squamous cell carcinoma. Oncogene 2000, 19: 2398–403. 10.1038/sj.onc.1203535PubMedView ArticleGoogle Scholar
- Yasumatsu R, Nakashima T, Hirakawa N: Maspin expression in stage I and II oral tongue squamous cell carcinoma. Head Neck 2001, 23: 962–6. 10.1002/hed.1139PubMedView ArticleGoogle Scholar
- Yoshizawa K, Nozaki S, Kitahara H, Kato K, Noguchi N, Kawashiri S, Yamamoto E: Expression of urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor and maspin in oral squamous cell carcinoma: Association with mode of invasion and clinicopathological factors. Oncol Rep 2011, 26(6):1555–60.PubMedGoogle Scholar
- Yoshizawa K, Nozaki S, Okamune A, Kitahara H, Ohara T, Kato K, Kawashiri S, Yamamoto E: Loss of maspin is a negative prognostic factor for invasion and metastasis in oral squamous cell carcinoma. J Oral Pathol Med 2009, 38(6):535–539. Epub 2009 Mar 13 Epub 2009 Mar 13 10.1111/j.1600-0714.2009.00762.xPubMedView ArticleGoogle Scholar
- Iezzi G, Piattelli A, Rubini C, Goteri G, Artese L, Perrotti V, Carinci F: Maspin expression in oral squamous cell carcinoma. J Craniofac Surg 2007, 18(5):1039–43. 10.1097/scs.0b013e31814b2a36PubMedView ArticleGoogle Scholar
- Marioni G, Gaio E, Giacomelli L, Bertolin A, D’Alessandro E, Stramare R, Facco E, Staffieri A, Blandamura S: MASPIN subcellular localization and expression in oral cavity squamous cell carcinoma. Eur Arch Otorhinolaryngol 2008, 265(Suppl 1):S97-S104. Epub 2008 Jan 31 Epub 2008 Jan 31PubMedView ArticleGoogle Scholar
- Marioni G, Blandamura S, Lionello M, Giacomelli L, Staffieri C, Fasanaro E, Manzato E, Staffieri A: Nuclear MASPIN expression relates to a better prognosis in elderly patients with laryngeal carcinoma. Acta Otolaryngol 2011, 131(11):1220–1225. Epub 2011 Jul 6 Epub 2011 Jul 6 10.3109/00016489.2011.599817PubMedView ArticleGoogle Scholar
- Ito Y, Yoshida H, Tomoda C, Uruno T, Takamura Y, Miya A, Kobayashi K, Matsuzuka F, Matsuura N, Kuma K, Miyauchi A: Maspin expression is directly associated with biological aggressiveness of thyroid carcinoma. Thyroid 2004, 14(1):13–8. 10.1089/105072504322783795PubMedView ArticleGoogle Scholar
- Boltze C, Schneider-Stock R, Meyer F, Peters B, Quednow C, Hoang-Vu C, Roessner A: Maspin in thyroid cancer: its relationship with p53 and clinical outcome. Oncol Rep 2003, 10(6):1783–7.PubMedGoogle Scholar
- Boltze C, Hoang-Vu C, Schneider-Stock R, Lehnert H, Roessner A: Role of the class II tumor suppressor gene maspin in thyroid carcinogenesis. Verh Dtsch Ges Pathol 2004, 88: 237–45.PubMedGoogle Scholar
- Tahany M, Shams MD, Rehab M, Samaka MD, Mohamed E, Shams MD: Maspin protein expression: a special feature of papillary thyroid carcinoma. Journal of the Egyptian Nat. Cancer Inst 2006, 18(3):274–280.Google Scholar
- Villares GJ, Zigler M, Dobroff AS, Wang H, Song R, Melnikova VO, Huang L, Braeuer RR, Bar-Eli M: Protease activated receptor-1 inhibits the Maspin tumor-suppressor gene to determine the melanoma metastatic phenotype. Proc Natl Acad Sci USA 2011, 108(2):626–631. 10.1073/pnas.1006886108PubMed CentralPubMedView ArticleGoogle Scholar
- Denk AE, Bettstetter M, Wild PJ, Hoek K, Bataille F, Dietmaier W, Bosserhoff AK: Loss of maspin expression contributes to a more invasive potential in malignant melanoma. Pigment Cell Res 2007, 20(2):112–9. 10.1111/j.1600-0749.2007.00363.xPubMedView ArticleGoogle Scholar
- Domann FE, Rice JC, Hendrix MJ, Futscher BW: Epigenetic silencing of maspin gene expression in human breast cancers. Int J Cancer 2000, 85(6):805–10. 10.1002/(SICI)1097-0215(20000315)85:6<805::AID-IJC12>3.0.CO;2-5PubMedView ArticleGoogle Scholar
- Futscher BW, Oshiro MM, Wozniak RJ, Holtan N, Hanigan CL, Duan H, Domann FE: Role for DNA methylation in the control of cell type specific maspin expression. Nat Genet 2002, 31(2):175–179. Epub 2002 May 20 Epub 2002 May 20 10.1038/ng886PubMedView ArticleGoogle Scholar
- Wada K, Maesawa C, Akasaka T, Masuda T: Aberrant expression of the maspin gene associated with epigenetic modification in melanoma cells. J Invest Dermatol 2004, 122(3):805–11. 10.1111/j.0022-202X.2004.22308.xPubMedView ArticleGoogle Scholar
- Chua R, Setzer S, Govindarajan B, Sexton D, Cohen C, Arbiser JL: Maspin expression, angiogenesis, prognostic parameters, and outcome in malignant melanoma. J Am Acad Dermatol 2009, 60(5):758–66. 10.1016/j.jaad.2009.01.018PubMedView ArticleGoogle Scholar
- Sabbatini R, Federico M, Morselli M, Depenni R, Cagossi K, Luppi M, Torelli G, Silingardi C: Detection of circulating tumor cells by reverse transcriptase polymerase chain reaction of maspin in patients with breast cancer undergoing conventional-dose chemotherapy. J Clin Oncol 2000, 18: 1914–1920.PubMedGoogle Scholar
- Ferrucci PF, Rabascio C, Mazzetta C: Mammaglobin expression in leukapheresis is a products predictive marker of poor prognosis in women high-risk with breast cancer. Clin Cancer Res 2004, 10: 6039–6046. 10.1158/1078-0432.CCR-03-0453PubMedView ArticleGoogle Scholar
- Leone F, Perissinotto E, Viale A, Cavalloni G, Taraglio S, Capaldi A, Piacibello W, Torchio B, Aglietta M: Tumour cell contamination detection of breast cancer cell contamination in leukapheresis product by real-time quantitative polymerase chain reaction. Bone Marrow Transplant 2001, 27: 517–523. 10.1038/sj.bmt.1702815PubMedView ArticleGoogle Scholar
- López-Guerrero JA, Gilabert PB, González EB, Sanz Alonso MA, Pérez JP, Talens AS, Oraval EA, de la Rubia CJ, Boix SB: Use of reverse-transcriptase polymerase chain reaction (RT-PCR) for carcinoembryonic antigen, cytokeratin 19, and maspin in the detection of tumor cells in leukapheresis products from patients with breast cancer: comparison with immunocytochemistry. J Hematother 1999, 8(1):53–61. 10.1089/106161299320578PubMedView ArticleGoogle Scholar
- Marioni G, Koussis H, Gaio E, Giacomelli L, Bertolin A, D’Alessandro E, Scola A, Ottaviano G, de Filippis C, Jirillo A, Staffieri A, Blandamura S: MASPIN’s prognostic role in patients with advanced head and neck carcinoma treated with primary chemotherapy (carboplatin plus vinorelbine) and radiotherapy: preliminary evidence. Acta Otolaryngol 2009, 129(7):786–92. 10.1080/00016480802412789PubMedView ArticleGoogle Scholar
- Beltran A, Parikh S, Liu Y, Cuevas BD, Johnson GL, Futscher BW: Re-activation of a dormant tumor suppressor gene maspin by designed transcription factors. Oncogene 2007, 26: 2791–2798. 10.1038/sj.onc.1210072PubMedView ArticleGoogle Scholar
- Beltran AS, Blancafor P: Reactivation of MASPIN in non-small cell lung carcinoma (NSCLC) cells by artificial transcription factors (ATFs). Epigenetics 2011, 6(2):224–235. 10.4161/epi.6.2.13700PubMed CentralPubMedView ArticleGoogle Scholar
- Zardo G, Fazi F, Travaglini L, Nervi C: Dynamic and reversibility of heterochromatic gene silencing in human disease. Cell Res 2005, 15: 679–690. 10.1038/sj.cr.7290337PubMedView ArticleGoogle Scholar
- Baylin SB, Ohm JE: Epigenetic gene silencing in cancer—a mechanism for early oncogenic pathway addiction? Nat Rev Cancer 2006, 6: 107–116.PubMedView ArticleGoogle Scholar
- Cameron EE, Bachman KE, Myohanen S, Herman JG, Baylin SB: Synergy of demethylation and histone deacetylase inhibition in the reexpression of genes silenced in cancer. Nat Genet 1999, 21: 103–107. 10.1038/5047PubMedView ArticleGoogle Scholar
- Yatabe Y, Mitsudomi T, Takahashi T: Maspin expression in normal lung and non-small-cell lung cancers: cellular property-associated expression under the control of promoter DNA methylation. Oncogene 2004, 23: 4041–4049. 10.1038/sj.onc.1207557PubMedView ArticleGoogle Scholar
- Hurtubise A, Momparler RL: Evaluation of antineo-plastic action of 5-aza-2′- deoxycytidine (Dacogen) and docetaxel (Taxotere) on human breast, lung and prostate carcinoma cell lines. Anticancer Drugs 2004, 15: 161–167.PubMedView ArticleGoogle Scholar
- Beltran AS, Sun X, Lizardi PM, Blancafort P: Reprogramming epigenetic silencing: artificial transcription factors synergize with chromatin remodeling drugs to reactivate the tumor suppressor mammary serine protease inhibitor. Mol Cancer Ther 2008, 7(5):1080–90. 10.1158/1535-7163.MCT-07-0526PubMed CentralPubMedView ArticleGoogle Scholar
- Villares GJ, Maya Z, Menashe B-E: The emerging role of the thrombin receptor (par-1) in melanoma metastasis - a possible therapeutic target. Oncotarget 2011, 2: 8–17.PubMed CentralPubMedView ArticleGoogle Scholar
- Santer FR, Malinowska K, Culig Z, Cavarretta IT: Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells. Endocr Relat Cancer 2010, 17(1):241–53. 10.1677/ERC-09-0200PubMed CentralPubMedView ArticleGoogle Scholar
- Schaefer JS, Zhang M: Targeting maspin in endothelial cells to induce cell apoptosis. Expert Opin Ther Targets 2006, 10(3):401–8. 10.1517/1472822.214.171.1241PubMedView ArticleGoogle Scholar
- Watanabe M, Nasu Y, Kashiwakura Y, Kusumi N, Tamayose K, Nagai A, Sasano T, Shimada T, Daida H, Kumon H: Adeno-associated virus 2-mediated intratumoral prostate cancer gene therapy: long-term maspin expression efficiently suppresses tumor growth. Hum Gene Ther 2005, 16(6):699–710. 10.1089/hum.2005.16.699PubMedView ArticleGoogle Scholar
- Strimpakos AS, Sharma RA: Curcumin: Preventive and Therapeutic Properties in Laboratory Studies and Clinical Trials. Antioxid Redox Signal 2008, 10(3):511–546. 10.1089/ars.2007.1769PubMedView ArticleGoogle Scholar
- Anand P, Sundaram C, Jhurani S, Kunnumakkara AB, Aggarwal BB: Curcumin and cancer: An “old-age” disease with an “age-old” solution. Cancer Lett 2008, 267: 133–164. 10.1016/j.canlet.2008.03.025PubMedView ArticleGoogle Scholar
- Prasad CP, Rath G, Mathur S, Bhatnagar D, Ralhan R: Potent growth suppressive activity of curcumin in human breast cancer cells: Modulation of Wnt/beta-catenin signaling. Chem Biol Interact 2009, 181(2):263–271. 10.1016/j.cbi.2009.06.012PubMedView ArticleGoogle Scholar
- Tsoli E, Tsantoulis PK, Papalambros A, Perunovic B, England D, Rawlands DA, Reynolds GM, Vlachodimitropoulos D, Morgan SL, Spiliopoulou CA, Athanasiou T, Gorgoulis VG: Simultaneous evaluation of maspin and CXCR4 in patients with breast cancer. J Clin Pathol 2007, 60(3):261–266.PubMed CentralPubMedView ArticleGoogle Scholar
- Reagan-Shaw S, Eggert D, Mukhtar H, Ahmad N: Antiproliferative effects of apple peel extract against cancer cells. Nutr Cancer 2010, 62(4):517–524. 10.1080/01635580903441253PubMedView ArticleGoogle Scholar
- Altenburg JD, Siddiqui RA: The Beneficial Effects of Nutritional Compounds on Breast Cancer Metastasis. In Breast Cancer Current and Alternative Therapeutic Modalities Edited by: Esra G. ISBN: 2011:978–953–307–776–5, InTech, Available from: http://www.intechopen.com/books/breast-cancer-currentand.
- Fremont L: Biological effects of resveratrol. Life Sci 2000, 66: 663–673. 10.1016/S0024-3205(99)00410-5PubMedView ArticleGoogle Scholar
- Maxwell S, Cruickshank A, Thorpe G: Red wine and antioxidant activity in serum. Lancet 1994, 344: 193–194.PubMedView ArticleGoogle Scholar
- Yu W, Fu YC, Wang W: Cellular and molecular effects of resveratrol in health and disease. J Cell Biochem 2011, 113: 752–759.View ArticleGoogle Scholar
- Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF: Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 1997, 275: 218–220. 10.1126/science.275.5297.218PubMedView ArticleGoogle Scholar
- Sheth S, Jajoo S, Kaur T, Mukherjea D, Sheehan K: Resveratrol reduces prostate cancer growth and metastasis by inhibiting the Akt/MicroRNA-21 Pathway. PLoS One 2012, 7(12):e51655. 10.1371/journal.pone.0051655PubMed CentralPubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.